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Theory of Endosymbiosis
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Eukaryotes emerged when mitochondria and chioroplasts (structures that formerly lived on their own) took up permanent residence inside other larger cells
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Modern Cell Theory
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all organisms composed of cells (schleiden and
Schwann)
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all cells arise from preexisting cells (Virchow
1855)
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Cells are the basic unit of all organisms
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Two types of cells
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Cells divided into two types
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Prokaryotes – no nucleus or other internal
membranes
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Eukaryotes – have nucleus and are more complex
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Prokaryotes vs. Eukaryotes
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No internal membranes, no nuclear membrane, E.R,
mitochondria, vacuoles, or other organelles vs. distinct organelles
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Circular, naked DNA vs. DNA wrapped with histone
proteins into chromosomes
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Small ribosomes vs. large ribosome’s
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Anaerobic or aerobic metabolism vs. aerobic
metabolism only
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No cytoskeleton vs. cytoskeleton
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Mainly unicellular vs. manily multicellular with
differentiation of cell types
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Small cells vs. larger cells
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Robert Hooke
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Developed first microscope
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Light microscopes vs. Electron microscopes
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Light microscope: uses light passing thru
specimen living or dead to form image
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Electron microscope: uses electrons passing
through specimen to form image
o
Transmission e- microscope: studies interior of
cells
o
Scanning e- microscopes: surface of cells
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Phase-contrast microscopes: used to examine
untainted, living cells
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Cell Fractionation
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ultracentrifuge used to separate liquid samples
at high speed based on differences in density to isolate desired layer
o
Pellet forms at bottom first rich in nuclei
o
Pellet forms at bottom then rich in mitochondria
o
Pellet forms at bottom finally rich in ribosomes
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Freeze fracture/Freeze Etching
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detailed cast of membrane. Tissue is then
digested way leaving only tissue cast and then examined under e-microscope
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Tissue culture
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Used to study properties of specific cells in viro (in lab);
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Differences in cell type
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Fat cells: long, distended, fat, round
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Nerve cells: long, thin, spindly because they
are supposed to transmit impulses across synapses
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Muscle cells: smooth cells
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Though they look different, they pretty much
have the same organelles
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Nucleus
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contains chromosomes (genetic material) and are wrapped with special
proteins into a chromatin network; surrounded by selectively permeable nuclear
membrane with pores that allow for transport of molecules (mRNA)
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Nucleolus
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Components of ribosomes are synthesized. One or two nucleoli may be
visible; not membrane-bound (tangle of chromatin)
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Ribosome
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sites of protein synthesis, found in cytoplasm or attached to
E.R.
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Endoplasmic Reticulum
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E.R: membranous system of channels and flattened sacs that traverse cytoplasm
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Types of E.R.
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Rough: site of protein synthesis
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Smooth has three functions:
o Assists in synthesis of steroied hormones and
other lipids
o Connects to rough e.r. to golgi apparatus
o Carries out various detox. Processes
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