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Step one
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The enzyme Hexokinase adds a P onto Glucose, by removing a P from ATP, yielding ADP and the next substrate Glucose-6-Phosphate.
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Step two
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The enzyme Phosphoglucoisomerase(Gly-2) rearranges the substrate Glucose-6-Phosphate into the next substrate in the reaction Fructose-6-phosphate.
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Step three
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The enzyme Phosphofructokinase-1 (Gly-3), adds another P group to Fructose-6-phosphate yielding Fructose-1-6-Phosphate and ADP.
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Step four
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The enzyme Aldolase (Gly-4) splits the substrate Fructose-1-6-Phosphate into Dihydroxyacetone and Glyceraldehyde-3-Phosphate.
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Step five
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The enzyme Triose phosphate isomerase changes Dihydroxyacetone phosphate into Glyceralehyde-3-Phosphate substrate. (2 PGALs now)
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Step six
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Enzyme Glyceraldehyde-3-Phosphate dehydrogenase adds a P to the PGALs & reduces NAD+ to NADH to yield 1,3-bisphosphoglycerate.
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Step seven
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Phosphoglycerokinase(Gly-7) removes a P from 1,3-bisphosphoglycerate adds it to ADP to yield ATP & 3-phosphoglycerate(substrate level Phos)
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Step eight
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Phosphoglyceromutase(Gly-8) rearranges the P on 3-phosphoglycerate to yield the substrate 2-phosphoglycerate.
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Step nine
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Enolase(Gly-9) removes a water from 2-phosphoglycerate to yield Phosphoenol-pyruvate(PEP), This has a C=C.
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Step ten
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Pyruvate Kinase(Gly-10) removes a phosphate and adds a H+ to Phosphoenol-pyruvate(PEP) to yield Pyruvate (2 Pyruvates/glucose). 1 ATP formed.
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