Biotech Test 2

Are small circular DNA molecules that replicate separately from the bacterial chromosome

Artificially constructed plasmids with a desired gene

Artificially constructed dna with desired genes inserted using sticky ends

The deliberate modification of the characteristics of an organism by manipulating its genetic material.

A virus that parasitizes a bacterium by infecting it and reproducing inside it. used to insert genes into cells

A DNA molecule that can carry foreign DNA into a host cell and replicate there

Cut DNA molecules at specific DNA sequences called restriction sites

A restriction enzyme usually makes many cuts, yielding

The most useful restriction enzymes cut DNA in a staggered way

Is a large plasmid that has been trimmed down and can carry a large DNA insert. another type of vector used in DNA library construction

Behave normally in mitosis and can carry more DNA than a plasmid as vectors helps avoid gene expression problems in eukaryotic cells

•made using bacteria is the collection of recombinant vector clones produced by cloning DNA fragments from an entire genome made using bacteriophages is stored as a collection of phage clones

A clone carrying the gene of interest can be identified with a

Proces used to determine if a clone has a desired trait. make complementary gene and see if it produces the protien

Method used to sequence dna where fragments are pulled through a porus gel and then read under a uv light