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Cards In This Set
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The process of DNA replication requires that each of the parental DNA strands be used as a _________ to produce a duplicate of the opposing strand.
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Template (this is a multiple-choice quesiton, not fill in the blank)
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The energy used by DNA polymeras to drive the elongation of a new DNA strand comes from...
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Release of pyrophosphate from an incoming deoxyribnucleotide with subsequent hydrolysis to inorganic phosphate.
(Warning: "all of the above" is a potential answer on this question...make sure not to chose it! : )
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If the genome of the bacterium E. coli requires about 20 minutes to replicate itself, how can the genome of the fruit fly Drosophila be replicated in only 3 minutes?
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Drosophila DNA contains more origins of replication than E. coli DNA.
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You have discovered an "Exo" mutant form of DNA polymerase in which the 3' -to-5' exonucleose function has been destroyed but the ability to join nucleotides together is unchanged. Which of the following properties do you expect the mutant polymerase to have?
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It will be more likely to generate mismatched base pairs.
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This topoisomerase works on areas where 2 double strands cross each other, forms a covalent linkage with both strands, makes a double-strand break, and reseals the break,
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DNA topoisomerase II
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All of the following statements regarding eukaryotic replication origins are true except...
A) replication occurs from many replication units; each unit containing 20-80 replication origins.
B) replication forks form in pairs and move in opposite directions from each other.
C) Initiatin occurs at one specific tim during S-phase
D) forks stop on meeting another advancing fork or when they reach the ends of chromosomes
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Initiation occurs at one specific time during S-phase
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Telomerase is a reverse transcriptase because it...
A) transcribes a DNA template in the 3' -> 5' direction.
B) transcribes a RNA template in the 3' -> 5' direction
C) makes an RNA strand from an RNA template.
D) makes a DNA strand from an RNA template
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Makes a DNA strand from an RNA template
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True or False: The enzymes involved in nucleotide excisionrpair are AP endonuclease and DNA glycosylases
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False
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A DNA synapsis:
A) is the basis of hybridization techniques used in the laboratory.
B) is facilitated by the action of RecA protein of E. coli.
C) is facilitated by Rad51 in humans
D) is necessary for strand invasion during homologous recombination.
E) all of the above
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E) all of the above
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True or False: Transpositional recombination is reversible.
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False
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What type of RNA blocks translation of selective mRNAs?
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MiRNA's (matching)
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What type of RNA serve as adaptors between mRNA and protein?
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TRNA (matching)
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What type of RNA codes for proteins?
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MRNA (matching)
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What type of RNA are parts of the spliceosome?
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SnRNAs (matching)
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What type of RNA catalyze protein synthesis?
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RRNA (matching)
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