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Cards In This Set
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Bacterial DNA Replication: Initiation. There are ____ bp in the oriC
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245
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What does the DnaA in E. Coli do?
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an initiator protein binds and unwinds a short section of DNA at oriC
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DNA helicase
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Unwinds and opens up a single strand region
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Prevents reannealing of ssDNA complementary strands
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Single-strand-binding protein (SSBs)
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DNA gyrase (topisomarase)
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Unwinds with double-stranded breaks and untwisting of the two strands
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Bacterial DNA Replication: Elongation. DNA Polymerase
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The enzymes that synthesize DNA. Adds a trinucleotide to only the 3' of the growing strnad and so new DNA strands always elongate in the same 5' to 3' direction.
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During the elongation of the Bacterial replication, primase
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Will lay down an RNA primer.
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The two single-stranded DNA template are
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Anti-parallel
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Elongation is always
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5' to 3'
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As the DNA is unwinding and the template strand that is exposed in the 3' to 5' direction allows the new strand to be synthesized continuously in the 5' to 3' direction. This new strand, thich undergoes continuous replication, is called
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The leading strand
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The newly made strand that undergoes discontinuous replication is called
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The lagging strand
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The short lengths of DNA produced by discontinuous replication of the lagging strand are called
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Okazaki fragments
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Discontinous replication is a result of which property of DNA?
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Charged phosphate group
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This DNA polymerase removes and replaces primers
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DNA polymerase I
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This DNA polymerase Elongates DNA
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DNA polymerase III
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