Glycolysis and Cellular Respiration

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How do autotrophs attain energy?
They use light energy to synthesize food molecules
How does the extraction of energy from food happen?
!) enzymes break large molecules into smaller ones (digestion) 2) other enzymes dismantle fragments extracting energy at each stage (catabolism)
What is glycoysis?
Conversion of glucose to pyruvate followed by cellular respiration (conversion of pyruvate to carbon dioxide and water in the presence of O2) which results in a release of large amounts of energy glucose + oxygen --> carbon dioxide + water + energy(ATP)
Know the chart for the Stages in the extraction of energy from foodstuffs
.
How do cells synthesize ATP?
Substrate-level phosphorylation
and
chemiosmosis - the majority is made this way in the inner mitochondrial membrane -- requires O2

*The synthesis of ATP requires energy
Generation of ATP by substrate-level phosphorylation
Involves direct transfer of phosphate group to ADP from another molecule

-eg. phosphoenolpyruvate (PEP) contains a high energy phosphate bond (similar to ATP), which can be transferred enzymatically to ADP
What is reduction?
The gain of one or more electrons by an atom, ion, or molecule
What is oxidation
Loss of one or more electrons
What is a redox reaction (oxidation-reduction)?
The transfer of electrons
Do oxidation and reduction always occur together?
Yes!
What does NAD+ serve as?
An "electron shuttle"

-exists in 2 forms; oxidized NAD+ and reduced NADH + H+

-functions as a coenzyme with dehydrogenases (specific type of enzyme)

-energy is harvested from glucose molecules in gradual steps using NAD+
Figure 9.3
.
The Respiratory Chain
ATP formation in the mitochondria results from the flow of electrons through this.

-NADH molecules transfer electrons to a series of membrane protein complexes (NADH Q reductase, cytochrome reductase and cytochrome oxidase)

-in conjunction with electron transport down the respiratory chain, protons are pumped across the membrane from the matrix
Chemiosmosis
The energy of the proton gradient across the mitochondrial membrane is used to drive ATP synthesis
ATP synthase
Returns protons to the matrix coupled with the synthesis of ATP