GREEN WORLD BLUE PLANET Quiz #1

33 cards   |   Total Attempts: 182
  

Cards In This Set

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Genetic Engineering
The transfer of genetic information to an organism to introduce a desirable trait
Phenotypes
Physical traits of an organism, inheritable.
Genotypes:
The genetic makeup of an organism
Genetic information is on...
Chromosomes, genes make up chromosomes, code for one trait
Prokaryotic
“Simplest”forms of life, Bacteria Cyanobacteria, Lack complexity of Eukaryotes
Eukaryotic
More Organized and complex, Organelles- membrane bound structures such as Nucleus, chloroplasts, mitochondria
Mitosis
Cell division to produce identical daughter cells
Meiosis
Cell division to produce gametes, haploid cells
Totipotency
All cells contain same genetic information
DNA- review structure of molecule
  • Nucleotides- Sugar, phosphate and a nitrogenous base
  • - Double stranded molecule
  • - Adenine, Guanine- Purines, Cytosine and Thymine-Pyrimidines
  • - Base Pairing is key- A-T and C-G links strands
  • Sequence of bases down strand is the key- information for coding proteins
Semi-conservative Replication
  • one strand retained when DNA is copied
Chromosomes must be duplicated prior to mitosis
Transcription
Make mRNA from DNA
Translation
At Ribosomes, use tRNA to make protein- review processes
  • Synthesis of protein via transfer RNA (tRNA)
  • 3 base pairs codes for one Amino acid, called a codon
  • Protein- a chain of Amino Acids
  • Occurs out in the cytoplasm at ribosomes
  • mRNA travels from nucleus to cytoplasm
Control of Gene Expression- Transcription factors and enhancers, different types of promoters
Promoter regions of genes- Enhancers and Transcription factors
DNA coiling- exposing gene for transcription- histone proteins
Micro RNA’s
Differential Splicing
Promoter regions of genes can determine whether or not a gene is activated
Enhancers and Transcription factors
Enhancers: regions of DNA to which transcription factors bind
Transcription Factors are proteins
Regulatory genes code for transcription factors that activate other genes
Promoters can be constituative- always on
Promoters can become active at stages of development
Can become active under certain environmental conditions
Can be useful for genetic engineering
Making and probing Genomic Library
  • Cut DNA into lots of pieces
  • Insert Pieces into plasmids
  • Insert plasmids into Bacteria
  • Grow Bacteria and Multiply fragments
  • Probe cloned fragments to find your gene