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Cards In This Set
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Histology
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the study of tissues of the body and how the tissues are arranged to contitute organs
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What are the main two components of tissues?
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Cells & extracellular matrix
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What is the extracellular matrix?
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filler that is composed of organized collagen fibrils and basement membranes
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What are the functions of the extracellular matrix?
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to furnish mechanical support for the cells
transport nutrients to the cells
carry away catabolites and secretory products
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What are potential problems when preparing tissues?
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distortions
loss of components due to the prep process
artifacts
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What is an example of a distortion?
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The compression of lumens
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What kind of components could be lost in the preparation process?
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Lipids could be lost due to the fact they dissolve in solvents
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What is an artifact?
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Things that occur in a tissue slide that aren't supposed to be there
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What are the 8 steps for preparing tissues to study?
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1. Collect tissue
2. Cut into small pieces, about 1 mm3
3. Fixation
4. Dehydration
5. Inflitration & Evaporation (Clearing)
6. Embedding
7. Slicing
8. Mounting
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What stops the metabolic processes that break down tissues?
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Fixation
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What is the purpose of fixing a tissue?
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to make the section of tissue permanent
to perserve the structure and molecular composition
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Which two processes can be used to fix a tissue?
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Chemical and physical
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What are examples of chemical fixation?
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formalin
formaldehyde & glutaraldehyde
glutaraldehyde
osmium tetroxide
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What is an example of physical fixation?
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Freezing
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What is formalin?
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A buffered isotonic solution of 37% formaldehyde
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