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Related Flashcards
Cards In This Set
| Front | Back |
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Which organelles can be seen with light microscopy?
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Nucleus, golgi (does not stain with H and E), nucleolus, centriole, mitochondria (with special stain)
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Hematoxylin and eosin commonly called...
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H and E
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3 steps of tissue preparation
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Fixation, infiltration with paraffin, staining
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4 purposes of fixation
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Terminate cell metabolism
prevent autolysis
kill viruses, bacteria, fungi
harden tissue (cross-link or denature proteins)
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PH during fixation
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Neutral
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Of hematoxylin and eosin - which is water-soluble and which is alcohol-soluble?
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Hematoxylin - water soluble
eosin - alcohol soluble
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Are basement membranes, intra-cellular lipids, elastic connective tissue, and reticular fibers seen with H and E?
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No
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What does periodic acid Schiff (PAS) stain?
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Basement membranes (purple)
glycogen (magenta)
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What does masson's or mallory's stain show?
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Muscle (red)
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What is trichrome stain for?
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Collagen (blue)
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What is weigert's stain for?
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Elastic fibers (black)
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What is silver stain for?
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Reticular fibers (black)
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What is oil red O stain for?
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Lipid (red)
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What is wright's or wright-giemsa stain for?
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Blood cells, cytology
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What is toluidine blue (T-blue) for?
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Mast cell granules
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