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Cards In This Set
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Small circular molecules of DNA in bacteria are called
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Plasmids
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Enzymes used to cut DNA molecules in recombinant DNA research are
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Restriction enzymes
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The fragments of chromosomes split by restriction enzymes
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Have specific sequences of nucleotides and sticky ends
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Restriction enzymes
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A. often produce staggered cuts in DNA that are useful in splicing genes.
b. are like most enzymes in being very specific in their action. c. are natural defense mechanisms evolved in bacteria to guard against or counteract bacteriophages. d. are used along with ligase and plasmids to produce a DNA library. e. all of these |
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Because it has no introns, researchers prefer to use __________ when working with human genes.
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CDNA
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DNA fragments result when _____ cut DNA molecules at specific sites.
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Restriction enzymes
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Foreign DNA that was inserted into a plasmid and then replicated many times in a population of bacteria is a _____
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DNA clone
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By reverse transcription, _____ is assembled on _____ .
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CDNA, mRNA
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RNA can manufacture DNA via the action of
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Reverse transcriptase
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PCR stands for
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Polymerase chain reaction
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Automated DNA sequencing relies on _____ .
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A. supplies of standard and labeled nucleotides
b. primers and DNA polymerases c. gel electrophoresis and a laser beam d. all of the above |
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The use of RFLPs for "genetic fingerprinting" is based on
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Differences of locations where enzymes make their cuts
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The laboratory technique used to separate the DNA fragments produced by automated DNA sequencing is
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Gel electrophoresis
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The DNA fragments produced by automated DNA sequencing are identified using
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Laser beams
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By gel electrophoresis, fragments of a gene library can be separated according to _____ .
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Length
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