Metcalf Micro Lab Practical 2

Includes Quiz Question from 4/16/2009


I also took it upon me to make both the question and the answer a question---because one can never be too sure if they are learning the material or just memorizing the answer! BOLD is the answer.

93 cards   |   Total Attempts: 188
  

Cards In This Set

Front Back
Two chemicals that can be added to a broth medium that tie up oxygen and contribute to an anaerobic environment AKA help to maintain a low redox potential.
Thioglycollate + L-cystine help anaerobes by ____.
(genus) grows very well in candle jar.
Acinobacter grows _____ in candle jar.
(genus) won't grow at all in candle jar.
Clostridium _____ in candle jar.
(genus) that will grow in anaerobic environment in a nutrient agar.
Clostridium is an _______.
(genus) that will not grow at all in anaerobic environment in a nutrient agar.
Acinobacter is an ______.
UV light has a wavelength of ______.
_______ has a wavelength of 265.
UV light can kill bacteria because of how it effects ______ component of bacteria.
_____ can kill bacteria because of how it effects pirimidine dimers (esp. thymine) on DNA.
Why shold food workers wash their hands?
______ helps to remove environmental bacteria even though it cannot destroy/remove our normal flora.
Why do phage plagues not continue to enlarge after the initial 1 day of incubation?
_____ needs growing bacteria (E. coli) to grow and therefore the ________ of incubation is all the time it gets to grow.
What substrate in Colilert broth do all coliform bacteria metabolize?
In ______, the substrate that all coliforms metabolize is. ONPG
ONPG is metabolized by (enzyme)
Beta-galactosidase metabolizes _____ in Colilert broth.
What substrate in Colilart broth does only E. coli metabolize?
Mug is metabolized by (organism).
Beta-glucoronidase metabolizes _____ in Colilert broth
Mug is metabolized by (enzyme).
On Petrifilm, what does E. coli (first picture) look like as opposed to all other coliforms (second picture) ?
Red with gas; Blue with gas.
Lactose Fermentation causes ______ to form in the Petrifilm test.
_______ causes gas bubbles to form in the Petrifilm test.