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Cards In This Set
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Streaking for isolation is a counting method, T/F?
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False, it is a way to get single, isolated colonies
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List 3 purposes of streaking for isolation
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Obtaining a pure colony
separating mixed cultures into individual colonies to study individual colony characteristics |
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All on plate are 1 kind of bacteria
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Pure Culture
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Average time for 1 bacterium to become 2 is ____, and after ____ generations in _____ hrs there is a pile of cells referred to as a ______
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20 minutes / 50-72 generations / 24hrs / Colony
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Colonies visible to the naked eye need to have how many cells?
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1 billion
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How is a pure colony different than a pure culture?
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Pure colonies are genetically identical (arising from single 'mother cell'). Pure cultures, though the same bacteria, arise from multiple mother cells and are not genetically identical.
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Culture that contains more than one type of bacteria
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Mixed Culture
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2 methods in micro to obtain pure culture from a mixed culture
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Streak plate and Pour plate methods
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Streaking for isolation will result in forming _____
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Pure colonies
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An individual isolated colony was started by one bacterial cell, mother cell, known as a ____
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Colony-Forming Unit (CFU)
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Pure colonies are used to start large stock culture called a ____
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Subculture
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Another term for 'pour method'
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Spread Method
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What does the Pour Method determine that the Streak Method does not?
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Number of cells in original culture
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Streaking for isolation is basically a method of ____
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Dilution
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The sample that you begin with is referred to as the ____
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Original Inoculum
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