Prepare for Microbiology Final Practical with Flashcards

Prepare for Microbiology Final practical briefly with these flashcards and revise the concepts. Learn the essential terms, definitions, and much more briefly with these flashcards quizzes.

25 cards   |   Total Attempts: 184
  

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Cards In This Set

Front Back
Question 1
  • Positive CAMP Test
  • Arrowhead zone of clearing, Streptococcus agalactiae acts with beta-hemolysin of Staph aureus
Question 2
  • DNA Hydrolysis
  • Clearing in agar DNAse present +
  • No clearing DNAse absent -
Question 3
  • SIM Sulfur reduction
  • Left tube H2S negative
  • Right tube H2S positive
Question 4
  • Urea broth
  • Pink rapid urea hydrolysis; strong urease production +
  • Orange or yellow No urea hydrolysis; organism does not produce urease or cannot live in broth -
Question 5
Decarboxylation No color change no decarboxylation -Yellow Fermentation; no decarboxylation -Purple Decarboxulation; specific decarboxylase enzyme +Removes carboxyl amino group
Question 6
Vogues-ProskauerRed + butanediol fermentation aceoin produced +No color change butanediol fermentaion acetoin is not produced +
Question 7
  • Oxidation/Fermentation
  • Mineral oil added for anerobic
  • Green or blue Oxidation O
  • Yellow Oxidation and fermentation or fermentation only O-F or F
  • Slight yellow at top Oxidation and slow fermentation or slow fermentation O-R or F
  • Green or blue No sugar metabolism; organism is nonsaccharolytic N
Question 8
Catalase Bubbles catalase +No bubbles Catalase -
Question 9
  • Gas(non-fermenter) Denitrification-production of NO3 to NO2 N2
  • Gas fermenter or status unknown Requires additional reagents
  • Red after reagents A and B Nitrate reduction to nitrite NO3 to NO2
  • No color change after reagents A and B Incomplete-add zinc
  • No color after zinc Nitrate reduction to nongaseous nitrogenous compounds
  • Red after zinc No nitrate reduction
Question 10
IndoleKovac reagentRed ring indole +No red indole -
Question 11
Gamma hemolysis
Question 12
Beta hemolysis
Question 13
Alpha hemolysis
Question 14
Citrate utilizationBlue Citrate is utilized +No color change; growth citrate is utilized +No color change, no growth citrate is not utilized -
Question 15
Oxidase
  • Dark blue/purple cytochrome c oxidase is present
  • No color change in 20 sec Cytochrome c oxidase not present