Module 15 Lab 17 Enterotube II

21 cards   |   Total Attempts: 182
  

Cards In This Set

Front Back
A single piece unit that may run from 10 to over 100 tests as the same time?
Multitest system
Number of compartments in the Enterotube?
12
What does each of the 12 compartments contain a different one of?
Medium
What are some of the compartments in the Enterotube covered with to generate Anaerobic conditions?
Wax
What is the reaction most frequently missed by students?
The gas reaction in the first compartment
Which end of the tube is the handle end of the tube?
The blue cap end, this contains the wire that should be bent
Should the white capped end wire be bend or straight?
Straight, this is the needle end of the tube
Do we touch this white end of the wire?
No, and we do not flame it either
What end of the tube do we use to scrape bacteria from the plate?
The white cap end which is the needle end
Do we completely pull the needle through from the tube?
No, not completely
What will we use to puncture holes in the 8 plastic slots in the enterotube?
The broken wire
How do you obtain the five digit number on the Entertube?
Circle the positive numbers then add them up
In the glucose compartment what 2 results will you read?
Yellow postive for glucose fermentation and Small bubbles in the agar or a gap between the agar and the wax is a positive result for gas production
In the Lysine and ornithine compartments, what color indicates a positive reaction (decarboxylation)?
Purple
In the Hydrogen Sulfide/Indole compartment what 2 results will you read?
Black color forming around the wire in the center is positive for the production of H2S. If the reagent turns bright red after adding the indole reagent its a positve reaction for indole from tryptophan