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Cards In This Set
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What is casein?
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Large globular protein that gives milk its white, opaque color
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As a protein, what is casein a good source of?
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Amino acids (carbon and nitrogen)
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Can casein molecules be transported across cell membranes intact?
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No, too big
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How do they get transported into the cell, what must happen first?
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They must be broken into subunits, amino acids or small peptides first
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What is Casease?
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An exoenzyme that is secreted outside of the cell that hydrolyzes casein to amino acids and short peptides
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What is the formula for casease production?
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Casein+Water+Amino acids
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How is casease production detected?
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By observing the agar surrounding a colony on as casein or milk agar plate
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How are the plates made?
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By adding skim milk (powder or liquid) to a general growth agar such as trypticase soy sugar
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What do the uninoculated plates look like?
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Opaque and white
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If an organism produces and secretes casease what does the plate look like?
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The casein in the agar surrounding the colony will be hydrolyzed and a clear zone will surround the colony
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If the organism does not produce casease what will the plate look like?
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The colony growth will appear on a white background
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How long do we incubate for?
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24 to 48 hours
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Casein is a protein that gives what its white color?
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Milk
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What kind of plates do we use for this exercise?
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Casein or milk agar plates to which evaporated skim milk is added
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Some organims produce enzyme casease, a protease exoenzyme. If the enzyme is produced and secreted into the agar what is broken down into smaller peptides and amino acids?
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Casein
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