Molecular Genetics

Flash cards for my molecular genetics test 

97 cards   |   Total Attempts: 182
  

Cards In This Set

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What is Helicase?
It is used to unwind the double stranded helix
What is SSBP?
It is the single stranded binding protein. it is used to prevent the single stranded DNA from reattaching
What is DNA polymerase III?
It synthisizes new DNA by attaching free nucelotides to the 3' end.
Which direction does DNA polymerase III move?
5' to 3'
What is primase?
It is responsible for building the RNA primers to attach the single stranded DNA
What is DNA polymerase I?
Removes DNA and replaces it with RNA
What is ligsase?
Attaches the 3' OH group to the 5' phosphate group in the lagging strand
What is mRNA complimentary to?
The DNA sequence
What is a double helix?
Two strands that are in the opposite directions
Why is DNA replicated?
So that resulting daughter cells after the cell has been divided contain equal amounts of DNA
What is step 1 of DNA replication?
Helicase splits up the double stranded DNA molecule forming two single stranded templates.
What is step 2 of DNA replication?
Soecial binding proteins known as SSBP attach to DNA to prevent them from renealing.
What is step 3 of DNA replication?
DNA polymerase attaches to ne strand, moves along it an joins incoming nucleotides together 5' to 3'
What is step 3 of DNA replication (lagging strand)?
Lagging strand (3' to 5') allows nucleotides to be synthesized is a discontinuous pattern. RNA primase synthesizes RNA primers then a DNA polymerase joins incoming nucleotides, and it stops where is meets another RNA primer
What is step 4 of DNA replication?
Enzyme Rnase H comes and removes the RNA primer on the lagging strand, leaving okazaki fragments.