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Streptococci and Enterococci are all catalase negative
Streptococci:
Three most important species of streptococci:
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Streptococci: catalase negative
1. Strep pyogenes.(Group A)
2. Strep agalactae (Group B)
3.Strep pneumoniae.
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Streptococci: catalase negative
gram stain:
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Streptococci: catalase negative
Gram Positive slightly smaller than staph, typically in chains.
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Streptococci: catalase negative
Catalase:
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Streptococci: catalase negative
Catalase negative as are all strep.
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Streptococci: catalase negative
Motility:
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Streptococci: catalase negative
Non-motile.
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Streptococci: catalase negative
Define pyogenic strep:
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Streptococci: catalase negative
Causes purelent infections.
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Streptococci: catalase negative
Primary charactistic of streptococci:
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Streptococci: catalase negative
Presence of lancefield antigens A-T
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Streptococci: catalase negative:
THREE MAJOR PRINCIPLES OF LANCEFIELD GROUPING:
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Streptococci: catalase negative
1. Carbohydrate antigens in cell wall extracts.
2. If beta hemolytic then the organism will be in one of the lancefield groups.
3. If the organism is able to be lancefield typed, then it may show alpha beta or gamma hemolysis.
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Streptococci: catalase negative
Which lancefield groups are the most common.
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Streptococci: catalase negative
'Most common types ar A. B, C, D, F, and G.
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Streptococci: catalase negative
Which lancefield groups are the most pathogenically significant.
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Streptococci: catalase negative
Groups A and B.
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Group A streptococci:
Typical type of hemolysis of group A strep.
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Group A streptococci:
Beta , Definite zone, Clear, Large in proportion to size of colony.
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Group A streptococci:
Two major hemolysins causing hemolysis.
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Group A streptococci:
1. Streptolysin S ( oxygen stabile)
2. Streptolysin O (oxygen Labile)
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Group A streptococci:
Typical differences in hemolysis under anaerobic and aerobic conditions with two major hemolsins
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Group A streptococci:
If streplysin S is not present , then you see hemolysis only under anaerobic conditions.
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Group A streptococci:
Two major substances that involve typing of group A strep.
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Group A streptococci:
With lancefield grouping all group A Streptococci have
1. rhamnose and n-acetly glucosamine (carbohydrarte).
2. Group A strep can be further classified by a M protien (present on the cell surfae) theere are approximatly 80 M protein serotypes.
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Group A streptococci:
Susceptibile to bacatracin :
PYR positive
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Group A streptococci:bacitracin sus , PYR Pos.
bacitracin sus
PTR pos.
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Group A streptococci:bacitracin sus , PYR Pos.
Major role of M antigen in immunity of grp A
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Group A streptococci:bacitracin sus , PYR Pos.
Increased resistance to phagocytosis Antibody toward particular M protein is protective.
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